Speaker
Jan H Driller
(Aarhus University)
Description
Mammalian membrane proteins are notoriously difficult to express. In contrast to bacteria or yeast, the baculovirus/insect cells system provides a good alternative to express these proteins in higher eukaryotes. Insect cells provide post-translational modifications, such as disulfide bonds or glycosylation, as well as a more akin membrane lipid composition compared to bacterial or yeast expression systems. Insect cells grow to high cell densities and using the baculovirus system even multi-protein complexes can be co-expressed.
