LINXS Event - Membrane Protein workshop: Structural Resolution of Membrane Proteins: From Expression to Sample Preparation

Session

Session 1 - Chair: Erika Toth

25 May 2021, 12:35

24. Keynote talk - Recombinant membrane protein production in microbial hosts

Roslyn Bill (Aston University, UK)

25/05/2021, 12:35

Despite many high-profile successes, recombinant membrane protein production remains a technical challenge; it is still the case that many fewer membrane protein structures have
been published than those of soluble proteins. However, progress is being made because empirical methods have been developed to produce the required quantity and quality of
these challenging targets. Microbial...

25. Contr. talk - The baculovirus/insect cells system

Jan H Driller (Aarhus University)

25/05/2021, 13:15

Mammalian membrane proteins are notoriously difficult to express. In contrast to bacteria or yeast, the baculovirus/insect cells system provides a good alternative to express these proteins in higher eukaryotes. Insect cells provide post-translational modifications, such as disulfide bonds or glycosylation, as well as a more akin membrane lipid composition compared to bacterial or yeast...

26. Contr. talk - Aquaporins – expression, purification and characterization

Kristina Hedfalk

25/05/2021, 13:35

Aquaporin water channels facilitate the bi-directional flow of water and small, neutral solutes down an osmotic gradient in all kingdoms of life. Over the last two decades, the availability of
high-quality protein has underpinned progress in the structural and functional characterization of these water channels. In particular, recombinant protein technology has guaranteed the supply of...