Name: LINXS Event - Membrane Protein workshop: Structural Resolution of Membrane Proteins: From Expression to Sample Preparation
Start: 2021-05-25T12:30:00+02:00
End: 2021-05-26T12:45:00+02:00
Location: No location set

LINXS Event - Membrane Protein workshop: Structural Resolution of Membrane Proteins: From Expression to Sample Preparation

from Tuesday 25 May 2021 (12:30) to Wednesday 26 May 2021 (12:45)

Monday 24 May 2021
Tuesday 25 May 2021

12:30 Welcome and purpose of the workshop
Welcome and purpose of the workshop
12:30 - 12:35
12:35 Session 1 - Chair: Erika Toth
12:35 - 13:55
Contributions

12:35 Keynote talk - Recombinant membrane protein production in microbial hosts - Roslyn Bill (Aston University, UK)

13:15 Contr. talk - The baculovirus/insect cells system - Jan H Driller (Aarhus University)

13:35 Contr. talk - Aquaporins – expression, purification and characterization - Kristina Hedfalk
13:55 Coffee Break
Coffee Break
13:55 - 14:00
14:00 Session 2 - Chair: Susanna Horsefield
14:00 - 15:20
Contributions

14:00 Keynote talk - An yeast GFP-based platform for functional and structures investigation of SLC transporters - David Drew (Stockholm University, Sweden)

14:40 Contr. talk - Study of anti-cancer effects of TTA-A2 and paclitaxel due to antagonistic interactions with T-type calcium channels - Vikram Dalal (Washington University in St. Louis)

15:00 Contr. talk - Production of insect odorant receptors in yeast for structural studies - Cassie Sims (Lund University)
15:20 Coffee Break
Coffee Break
15:20 - 15:30
15:30 Session 3 - Chair: Maria Gourdon
15:30 - 16:30
Contributions

15:30 Keynote talk - Preparation of lipid flippases for cryo-EM studies - Joseph Lyons (Aarhus University, Denmark)

16:10 Contr. talk - Production and purification of the K+-dependent P-Type ATPase KdpFABC for crystallization and cryo-EM - Charlott Stock (Aarhus University)
16:30 Short Break
Short Break
16:30 - 16:35

16:35 General Discussion (Chair: Susanna Horsefield)
General Discussion (Chair: Susanna Horsefield)
16:35 - 17:00
Wednesday 26 May 2021
09:00 Session 4 - Chair: Karin Lindkvist
09:00 - 10:20
Contributions

09:00 Keynote talk - Membrane protein purification and application in drug discovery - Arjan Snijder (Astra Zeneca)

09:40 Contr. talk - Towards Neutron crystallography of membrane proteins: Insights into production of deuterium-labelled OmpF - Swati Aggarwal (European Spallation Source)

10:00 Contr. talk - Production of Bcl-2 proteins involved in regulation of mitochondrial apoptosis - Jörgen Ådén (Umeå University)
10:20 Coffee Break
Coffee Break
10:20 - 10:30
10:30 Session 5 - Chair: Swati Aggarwal
10:30 - 11:50
Contributions

10:30 Keynote talk - Whipping GPCRs into shape for structure determination - Christopher G. Tate (MRC Laboratory of Molecular Biology, UK)

11:10 Contr. talk - From expression to purified protein: a P4-ATPase for CryoEM studies - LineMarie Christiansen (Aarhus University)

11:30 Contr. talk - Small-scale expression and purification of the cytokine receptor, MPL, using the ALiCE® cell-free system - Julie Tucker (University of York)
11:50 Short Break
Short Break
11:50 - 11:55

11:55 General Discussion (Chair: Urban Johanson)
General Discussion (Chair: Urban Johanson)
11:55 - 12:20

12:20 Closing remarks, introducing the autumn workshop
Closing remarks, introducing the autumn workshop
12:20 - 12:25