LINXS Event - Membrane Protein workshop: Structural Resolution of Membrane Proteins: From Expression to Sample Preparation

Session

Session 2 - Chair: Susanna Horsefield

25 May 2021, 14:00

27. Keynote talk - An yeast GFP-based platform for functional and structures investigation of SLC transporters

David Drew (Stockholm University, Sweden)

25/05/2021, 14:00

Recombinant expression screening of eukaryotic membrane proteins for functional and structural investigation is still a trial-and-error process. We have found that cloning gene-strings by homologuous recombination into a 2u vector S. cerevisiae expression vector and then detecting membrane protein expression and stability by working with GFP-fusions, enables many constructs to be rapidly...

28. Contr. talk - Study of anti-cancer effects of TTA-A2 and paclitaxel due to antagonistic interactions with T-type calcium channels

Vikram Dalal (Washington University in St. Louis)

25/05/2021, 14:40

Studies have shown that in cancer cells, there is an increased T-type calcium channel (TTCC) expression compared to healthy cells. Therefore, the studies targeting TTCC for cancer therapy
have shown many positive outcomes. Here, we have used TTA-A2- a potent TTCC inhibitor as a test drug, and paclitaxel (PTX)- a tubule-binding anti-cancer agent as a positive control. Blocking
TTCC has shown to...

29. Contr. talk - Production of insect odorant receptors in yeast for structural studies

Cassie Sims (Lund University)

25/05/2021, 15:00

Insect olfactory receptors (ORs) are proteins involved in olfaction in insects. Insect ORs are 7-transmembrane domain proteins that possess a unique structure, forming a heteromeric complex with a highly conserved co-receptor (ORCO). The heteromeric complex constitutes an ion channel, which opens allowing movement of ions upon activation by a ligand. Specificity of ORs is variable, and little...