Over the past 15 years we have determined multiple structures of GPCRs by both X-ray crystallography and cryo-EM. For each GPCR we had to overcome the difficulties of overexpression, solubilisation, stabilisation and structure determination, as you would for any membrane protein. For X-ray crystallography, extensive protein engineering was required to form well-diffracting crystals, in...
The Saccharomyces cerevisiae P4-ATPase Neo1p is believed to function as a lipid flippase, translocating lipids towards the cytosolic leaflet of both Golgi and endosome membranes. In
order to screen around both functional and structural studies to ascertain the exact role and function of Neo1p an efficient high-yield expression and purification protocol is vital. By
overexpression in S....
MPL (also called TpoR) is the receptor for the haematopoietic cytokine, thrombopoietin (TPO). Together, MPL and TPO control the production of platelets and the maintenance of haematopoietic stem cells. Gain of function mutations in MPL constitute ˜5-10 % of driver mutations in essential
thrombocythemia and primary myelofibrosis, whilst loss of function mutations give rise to thrombocytopenias....
