In the presentation I will discuss our capabilities, processes and methods for membrane protein expression and purification, including our recently developed teabag purification method. The teabag method allows rapid purification of membrane proteins with reduced hands-on and processing time. Application of membrane proteins in drug discovery will be illustrated by our work on the...
Hydrogen bonds play a crucial role for protein function and involved in almost every mechanism from foundation of protein structure to enzyme catalysis. Hydrogen (1H) atoms form the basis of hydrogen bond that is not scattered by X-ray crystallography due to its poor scattering power. Neutron protein crystallography (NPX) is a powerful tool that is capable of locating hydrogens and study the...
Programmed cell death (apoptosis) is essential for human life. In its intrinsic pathway, the Bcl-2 (B-cell lymphoma 2) protein family regulates cell life and death by controlling permeability of the mitochondrial outer membrane (MOM). However, the molecular basis of cell protection by its anti-apoptotic Bcl-2 members remains elusive due to the lack of detailed structural insight into their...
