Recombinant expression screening of eukaryotic membrane proteins for functional and structural investigation is still a trial-and-error process. We have found that cloning gene-strings by homologuous recombination into a 2u vector S. cerevisiae expression vector and then detecting membrane protein expression and stability by working with GFP-fusions, enables many constructs to be rapidly...
Studies have shown that in cancer cells, there is an increased T-type calcium channel (TTCC) expression compared to healthy cells. Therefore, the studies targeting TTCC for cancer therapy
have shown many positive outcomes. Here, we have used TTA-A2- a potent TTCC inhibitor as a test drug, and paclitaxel (PTX)- a tubule-binding anti-cancer agent as a positive control. Blocking
TTCC has shown to...
Insect olfactory receptors (ORs) are proteins involved in olfaction in insects. Insect ORs are 7-transmembrane domain proteins that possess a unique structure, forming a heteromeric complex with a highly conserved co-receptor (ORCO). The heteromeric complex constitutes an ion channel, which opens allowing movement of ions upon activation by a ligand. Specificity of ORs is variable, and little...
