Despite many high-profile successes, recombinant membrane protein production remains a technical challenge; it is still the case that many fewer membrane protein structures have
been published than those of soluble proteins. However, progress is being made because empirical methods have been developed to produce the required quantity and quality of
these challenging targets. Microbial...
Mammalian membrane proteins are notoriously difficult to express. In contrast to bacteria or yeast, the baculovirus/insect cells system provides a good alternative to express these proteins in higher eukaryotes. Insect cells provide post-translational modifications, such as disulfide bonds or glycosylation, as well as a more akin membrane lipid composition compared to bacterial or yeast...
