Speaker
Charlott Stock
(Aarhus University)
Description
At very low extracellular K+ concentrations (<100 μM), KdpFABC rescues bacterial cells by taking up K+ against an up to 105-fold concentration gradient. To understand the mechanism of this heterotetrameric complex, KdpFABC was
purified for functional and structural investigations. A new expression system for KdpFABC was generated, a 3-step chromatographic purification implemented and protein functionality in different detergents as well as in the presence of
inhibitors was tested. The work was performed in the lab of Prof. Inga Hänelt at the University of Frankfurt from 2014-2020.
