Mitochondria contain approximately 1200 different proteins, 99% of which are synthesized on cytosolic ribosomes and need to be delivered into the right destination through the intermembrane space by transport machineries, such as the TIM chaperone. Currently, the mechanistic and structural details of how the TIM chaperone binds to these mitochondrial proteins remain elusive. To gain structural...
In bacteria, tryptophan synthesis is performed by the enzymes encoded in the trp operon. The product of the
trpC gene, indole-3-glycerol phosphate synthase (IGPS) catalyzes the indole-forming reaction of tryptophan
synthesis. The reaction mechanism includes a decarboxylation step of the substrate 1-(o-carboxyphenylamino)
1-deoxyribulose 5-phosphate (CdRP). The decarboxylation has been assumed...
Structural studies of integral membrane proteins (IMPs) are challenging, as many of them are inactive or insoluble in the absence of a lipid environment. We pioneered an approach making use of fractionally deuterium labelled ‘stealth carrier’ nanodiscs that are effectively invisible to low-resolution neutron diffraction and enable structural studies of IMPs in a lipidic native-like solution...
