Niemann-Pick type C (NPC) proteins are essential for sterol homeostasis, believed to drive sterol integration into the vacuolar/lysosomal membrane before redistribution to other cellular membranes. Using a combination of crystallography, cryo-electronmicroscopy, biochemical and in vivo studies on the Saccharomyces cerevisiae NPC system, NCR1/NPC2, we recently generated a framework for sterol...
Small-angle X-ray scattering (SAXS) is a powerful method in the studies of solutions of biological macromolecules and nanostructured systems [1] allowing one to analyze the structure of native particles and complexes and to rapidly assess structural changes in response to variations in external conditions. Dedicated high brilliance synchrotron beamlines and novel data analysis methods [2]...
The genetic code of viruses, RNA or DNA, are typically protected in an icosahedral capsid, which is primarily
assembled from over a hundred subunits of the same protein in a spontaneous self-assembly process. Similar
highly efficient assembly processes are ubiquitous in biological systems; viral capsids present a unique platform
to exploit for therapeutic advances in the targeted cellular...
