Using membrane protein crystallography, small-angle scattering techniques, and cryo-EM, and also a range of biochemical and biophysical methods such as electrophysiology, single-molecule FRET, and molecular dynamics simulations, we have obtained deep insight into the functional cycle of primary active transporters of the P-type ATPase family. These transport ATPases are fundamental to...
The multivesicular sorting machinery is a crucial mechanism for targeting membrane proteins
for recycling or degradation. The lysosomal trafficking regulator-interacting protein 5 (LIP5)
which coordinates the action of this machinery is also known to bind directly to the membrane
protein cargo. In case of aquaporin 2 (AQP2) the binding of LIP5 during the endocytic pathway
in kidney collecting...
In my talk I will present our approach for modeling macromolecular
flexibility of large molecular assemblies and how it can be combined with
sparse experimental data obtained with small-angle and cross-linking
experiments.
Large macromolecular machines, such as proteins and their complexes, are
typically very flexible at physiological conditions, and this flexibility is
important for their...
With the technological breakthroughs in the past few years, single particle cryo-electron microscopy (cryo-EM) has enabled rapid progresses in structure determination of integral membrane proteins, particularly ion channels. The pace of structure determination of integral membrane proteins by single particle cryo-EM is unprecedented in structural biology. With such a rapid progress, it is also...
