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Dynamics of Biological Macromolecules

4–6 Jun 2018
Skissernas Museum
Europe/Stockholm timezone

Session

Dynamics of Proteins in Crowded and Confined Geometry

DPCCG
5 Jun 2018, 14:00
Skissernas Museum

Skissernas Museum

Finngatan 2 223 62 Lund Sweden

Presentation materials

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  1. 61. KEYNOTE 8 - Interplay of gating and hydrodynamic interactions in crowded protein solutions
    Prof. Jeffrey Skolnick (CSSB, Atlanta)
    05/06/2018, 14:00
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    An outstanding challenge in computational biophysics is the simulation of a living cell at molecular detail. Over the past several years, using Stokesian Dynamics, progress has been made in simulating coarse grained molecular models of the cytoplasm. Since macromolecules comprise 20-40% of the volume of a cell, one would expect that steric interactions dominate macromolecular diffusion....

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  2. 71. KEYNOTE 9 - Role of shape anisotropy in interpreting small angle X-ray scattering (SAXS) studies on concentrated protein solutions
    Prof. Robin Curtis (University of Manchester)
    05/06/2018, 14:40
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    There is a need for achieving high protein concentration liquid formulations of antibody therapeutics to meet patient dose requirements. Predicting the concentrated solution behaviour requires understanding how to map protein-protein interactions on simplified models, which account for the relative contributions from repulsive and attractive forces, shape and interaction anisotropy, and any...

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  3. 76. Contributed talk 5 - Rotational and translational diffusion of eye lens gamma crystallin at low and intermediate concentrations
    George Thurston (Rochester Institute of Technology)
    05/06/2018, 15:20
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    We are conducting continuing studies of rotational diffusion, translational diffusion, and thermodynamic compressibility of the eye lens protein bovine gammaB crystallin at low and intermediate protein concentrations. For nuclear magnetic resonance (NMR) measurements, 15N-labeled bovine gammaB crystallin was produced in transformed E Coli by recombinant means, and isolated using size-exclusion...

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  4. 73. KEYNOTE 10 - X-ray Photon Correlation Studies of Diffusion in Concentrated Protein Suspensions
    Prof. Laurence Lurio (Northern Illinois University)
    05/06/2018, 16:10
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    The talk will present an introduction to x-ray photon correlation spectroscopy (XPCS) and the specific issues associated with XPCS measurements on biological macromolecules. This will include flux requirements and methods to ameliorate beam damage. XPCS measurements of the dynamics of concentrated suspension of eye-lens proteins will be presented. The measured time correlation functions...

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  5. 65. Contributed talk 6 - Low radiation dose XPCS for dynamic studies of biological matter
    Prof. Christian Gutt (University Siegen)
    05/06/2018, 16:50
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    X-ray photon correlation spectroscopy (XPCS) measures nanoscale dynamics in real time by correlations of X-ray speckle patterns. The speckle patterns yield access to density-density correlation functions and also to higher order correlation functions. However, the highly intense X-ray beams of modern storage rings are also the cause for considerable radiation damage to the samples....

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  6. 72. KEYNOTE 12 - Hemoglobin diffusion and the dynamics of oxygen capture by the red blood cells
    Dr Stéphane Longeville (LLB Saclay)
    06/06/2018, 09:00
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    Translational diffusion of macromolecules in cell is generally assumed to be anomalous due high macromolecular crowding of the milieu. Red blood cells are a special case of cells filled quasi exclusively (95 % of the dry weight of the cell) with an almost spherical protein: hemoglobin. Hemoglobin diffusion has since a long time been recognized as facilitating the rate of oxygen diffusion...

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  7. 51. Contributed talk 7 - Influence of shape and interaction anisotropy on short-time protein diffusion
    Jin Suk Myung (Lund University)
    06/06/2018, 09:40
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    In a dense and crowded environment such as the cell, an individual protein feels the presence of surrounding proteins. It is thus expected that direct and hydrodynamic interactions strongly affect the diffusion of proteins. Examples are suspensions of eye lens proteins, where a dramatic slow down of the local short-time diffusion of γB-crystallin and a dynamical arrest is observed...

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  8. 53. Contributed talk 8 - Towards crowding in the eye lens: dynamics in aqueous solutions of crystallin proteins
    Felix Roosen-Runge
    06/06/2018, 10:00
    Dynamics of proteins in crowded and confined geometry
    Oral presentation

    The function of the eye is dependent on a transparent, optically refractive, and deformable eye lens. These specific physical properties are realized by a crowded multicomponent mixture of mainly crystallin proteins within the cells in the eye lens. The underlying biophysical mechanisms are not only of fundamental interest, but highly relevant to better understand and treat eye conditions such...

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  9. 33. Contributed Talk 10
    Dynamics of proteins in crowded and confined geometry
    Oral presentation
  10. 31. Contributed Talk 5: Rotational and translational diffusion of eye lens gamma crystallin at low and intermediate concentrations
    Prof. George Thurston (Rochester Institute of Technology)
    Dynamics of proteins in crowded and confined geometry
    Oral presentation
  11. 34. Contributed Talk 6: Low radiation dose XPCS for dynamic studies of biological matter
    Christian Gutt (University Siegen)
    Dynamics of proteins in crowded and confined geometry
    Oral presentation
  12. 78. Contributed Talk 7: Influence of shape and interaction anisotropy on short-time protein diffusion
    Jin Suk Myung (Lund University)
  13. 79. Contributed Talk 8: Towards crowding in the eye lens: dynamics in aqueous solutions of crystallin proteins
    Felix Roosen-Runge
  14. 32. KEYNOTE 10 - X-ray Photon Correlation Studies of Diffusion in Concentrated Protein Suspensions
    Prof. Laurence Lurio (Northern Illinois University)
    Dynamics of proteins in crowded and confined geometry
  15. 35. KEYNOTE 11 - Neutron scattering and drug discovery
    Prof. Jeremy Smith ( Oak Ridge National Laboratory)
    Dynamics of proteins in crowded and confined geometry
  16. 36. KEYNOTE 12 - Diffusion of oxygen transport proteins and the dynamics of O2 capture by red blood cells
    Dr Stéphane Longeville (LLB Saclay)
    Protein dynamics and drug discovery
  17. 29. KEYNOTE 8 - Interplay of gating and hydrodynamic interactions in crowded protein solutions
    Prof. Jeffrey Skolnick (CSSB, Atlanta)
    Dynamics of proteins in crowded and confined geometry
  18. 30. KEYNOTE 9 - Prediction of concentrated solution properties for monoclonal antibody solutions
    Prof. Robin Curtis (University of Manchester)
    Dynamics of proteins in crowded and confined geometry
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